The fresh new Single cell Study Core provides fresh and you may computational help getting single-cellphone RNA-Seq (scRNA-Seq) and you can unmarried-cellphone ATAC-Seq (scATAC-Seq)
Brand new Core premiered that have generous support about Columbia Reliability Medicine Effort, the latest Irving Institute to have Medical and Translational Look , the new Herbert Irving Comprehensive Cancer Cardio , plus the Department out of Medication . We’re delighted to provide several properties by using the 10x Genomics Platform: 3′ Single cell Gene Phrase, 5′ Single-cell Gene Phrase (as well as V/D/J sequencing), Single cell ATAC, and you may Single cell Multiome ATAC + Gene Term.
New 10x Genomics Single cell Gene Term 3′ and you can 5′ workflows try one another highest-throughput, scalable technologies one to encapsulate tens and thousands of personal cells (or nuclei) for the droplets to have lysis and contrary transcription out-of to 8 examples for the parallel. The latest droplets consist of primers to possess cell- and you will and you may molecule-certain barcoding from cDNA. We could prepare yourself libraries having Mention-Seq/function barcoding, CRISPR, otherwise mobile hashing. Having fun with all of our 5′ workflow, we could character a complete duration V(D)J regions out-of T-phone or B-cell receptors regarding human and you can mouse examples.